We quantified relative extent of pelage staining in Scalopus aquaticus (eastern mole) as an indicator of scent-gland marking, and evaluated whether staining was associated with colored pelage spots and patches often prevalent on the snout and ventral surface of individuals. Moles were collected from southern Illinois (n = 91) and from Cincinnati, OH (n = 152). Adult moles scent-marked more than juveniles, but pelage staining was independent of breeding season for males and females. Pelage spotting occurred in 33.7% of the sample and was not associated with pelage staining from glandular secretions, as has been suggested by some previous investigators. Pelage spots were most prevalent on the ventral surface. Ventral spotting occurred more often in males than females (P < 0.001). Mean area of ventral spots was 2.81 cm² with no differences in area related to sex or age.